Multidrug-Resistant IncA/C Plasmid in Vibrio cholerae from Haiti - Volume 20, Number 11—November 2014 - Emerging Infectious Disease journal - CDC

Multidrug-Resistant IncA/C Plasmid in Vibrio cholerae from Haiti - Volume 20, Number 11—November 2014 - Emerging Infectious Disease journal - CDC

Volume 20, Number 11—November 2014

Letter

Multidrug-Resistant IncA/C Plasmid in Vibrio cholerae from Haiti

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• Letter
• Suggested Citation

Tables

• Table

Technical Appendicies

• Technical Appendix

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• PDF[317 KB - 3 pgs]

Suggested citation for this article

To the Editor: The agents of epidemic cholera are Vibrio cholerae toxigenic serogroups O1 and O139. Cholera symptoms include watery diarrhea and severe dehydration, which can rapidly result in death unless rehydration therapy is prompt (1). Antimicrobial agents may reduce the severity and duration of disease (1); commonly used are tetracyclines, fluoroquinolones, macrolides, and trimethoprim/sulfamethoxazole (1). However, V. choleraeresistance to antimicrobial drugs is increasing because of the accumulation of genetic mutations and the acquisition of resistance genes, which are usually transferred on mobile genetic elements such as integrating conjugative elements (ICEs) (1).

As of March 12, 2014, the ongoing cholera outbreak that began in Haiti in October 2010 had caused 700,796 cases and 8,548 deaths (2). To characterize infections, the National Public Health Laboratory in Haiti and the US Centers for Disease Control and Prevention (CDC) collaborated to perform standard microbiological and antimicrobial-drug susceptibility testing on isolates from case-patients. Since October 2010, the National Public Health Laboratory has identified 465 isolates, which were then forwarded to CDC for determination of MICs for 15 antimicrobial agents by broth microdilution (Sensititer; Trek Diagnostics Systems, Cleveland, OH, USA) according to manufacturer’s recommendations (Table). Resistance was defined by the Clinical and Laboratory Standards Institute interpretive standards, when available (3). The typical outbreak strain (2010EL-1786) displayed resistance to streptomycin, sulfisoxazole, trimethoprim/sulfamethoxazole, and nalidixic acid, and decreased susceptibility to ciprofloxacin and chloramphenicol (4). Resistance was caused by mutations in the QRDR regions of the gyrA and parC genes and presence of ICEVchHai1 containing the dfrA1, floR, strAB, andsul2 resistance genes (4).

In April of 2012, the 2 agencies began sentinel laboratory-based surveillance for acute diarrheal disease at 4 hospitals in Haiti (5). As part of this surveillance, fecal specimens were sent to the National Public Health Laboratory for organism isolation, identification, antimicrobial-drug testing, and subsequently to CDC for expanded antimicrobial-drug testing and molecular characterization. One isolate, 2012EL-2176, showed the typical resistance phenotype of the outbreak strain but additional resistance to ampicillin, amoxicillin/clavulanic acid, cefoxitin, ceftriaxone, ceftiofur; the tetracycline MIC was intermediate (Table).

Analysis of this isolate by serotype, pulsed-field gel electrophoresis, multilocus variable number–tandem repeat analysis, and whole-genome sequencing confirmed that the isolate was similar to outbreak isolates (data not shown) (6). PCR and whole-genome sequencing analysis by use of ResFinder (http://www.genomicepidemiology.org/) identified the original outbreak resistance determinants and additional determinants (aac(3)-IIa, blaCMY-2,blaCTX-M-2, blaTEM-1, dfrA15, mphA, sul1, and tetA) (7). Plasmid transfer by electroporation into Escherichia coli (DH10B) confirmed that the resistance determinants were plasmid encoded. PCR-based replicon testing identified an IncA/C2 plasmid, and PCR and whole-genome sequencing confirmed that the plasmid encoded a unique set of resistance determinants (aac(3)-IIa, blaCMY-2, blaCTX-M-2, blaTEM-1, dfrA15, mphA, sul1, and tetA) and a second copy of the resistance genes floR, strAB, and sul2 identical to those located in ICEVchHai1 (8). Antimicrobial-drug susceptibility testing of the transformant demonstrated transfer of the resistance profile and additional resistance to chloramphenicol, tetracycline, and decreased susceptibility to azithromycin (Table). The lack of association between the presence of the resistance determinants floR and tetA and the lack of resistance in V. cholerae has been observed previously, possibly because of lower gene expression (9). The plasmid was mobilizable by conjugation (conjugation efficiency = 1.3–1.4 × 10−2) when E. coli J53 was used as the recipient.

IncA/C plasmids are widespread in Enterobacteriaceae and commonly confer multidrug resistance. BLASTn (http://blast.ncbi.nlm.nih.gov/Blast.cgi) comparison of the completed plasmid p2012EL-2176 sequence with the National Center for Biotechnology Information nucleotide collection showed similarities to other IncA/C-blaCMY plasmids; most similarity (total score = 3.009 × 105) was to pAR060302, found in an E. coli isolate from a dairy calf (10). Most IncA/C-blaCMY plasmids have 3 resistance regions: sul2 region (floR-tetA-strAB-sul2), cmy-2 insertion region, and Tn21-like region (aad-aac). Plasmid p2012EL-2176 contains the sul2 and cmy-2 insertion regions and a putative arr3-drfA27-aadA16-sul1 resistance gene cassette at the Tn-21 location. This plasmid has additional resistance gene insertions: a putative cassette containing a blaTEM-1 and aac(3)-IIa gene upstream of the sul2 region and insertions of blaCTX-M-2, sul1, and mphA genes downstream of the arr3-drfA27-aadA16-sul1 cassette (Technical Appendix[PDF - 121 KB - 2 pages]).

Since discovery of isolate 2012EL-2176, sentinel surveillance has not detected increased antimicrobial-drug resistance among V. cholerae in Haiti. The ability of IncA/C plasmids to acquire novel resistance cassettes from multiple sources makes it difficult to hypothesize as to the origins of plasmid p2012EL-2176. Although this plasmid was most closely related to a plasmid found in E. coli, it was also closely related to plasmids in Salmonella, Klebsiella, and Providencia. Enterobacteriaceae are found in the environment and/or in the host gut; therefore, the isolate could have acquired the plasmid in the environment or within the host. The latter scenario would limit the possible spread of this plasmid and could explain its rarity. The original Haiti outbreak isolate has been shown to be poorly naturally transformable, accounting for the lack of acquired chromosomal genes and nearly homologous genomic content among outbreak isolates (6). Therefore, the acquisition of plasmids, and their resistance genes, may represent the major source of future variability among V. cholerae involved in the Haiti outbreak.

Jason P. Folster , Lee Katz, Andre McCullough, Michele B. Parsons, Kristen Knipe, Scott A. Sammons, Jacques Boncy, Cheryl Lea Tarr, and Jean M. Whichard

Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J.P. Folster, L. Katz, M.B. Parsons, K. Knipe, S.A. Sammons, C.L. Tarr, J.M. Whichard); International Health Resources Consulting, Atlanta (A. McCullough); Laboratoire National de Santé Publique, Port-au-Prince, Haiti (J. Boncy)

Acknowledgment

We thank Deborah Talkington, Chery Bopp, Nancy Garrett, Maryann Turnsek, Emmanuel Rossignol, Nicole Freeman, PulseNet, and the Health Systems Recovery Team for their contributions to this work.

References

1. Harris JB, LaRocque RC, Qadri F, Ryan ET, Calderwood SB. Cholera. Lancet. 2012;379:2466–76. DOIPubMed
2. Republique d’Haiti Ministère de la Santé Publique et de la Population. Ministère de la Santé Publique et de la Population (MSPP) rapport de cas [cited 2014 Mar 18]. http://mspp.gouv.ht/site/downloads/Rapport%20Web_18.03_Avec_Courbes_Departementales.pdf.
3. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; twenty-first informational supplement. CLSI Document M100–S21. Wayne (PA): The Institute; 2013.
4. Sjölund-Karlsson M, Reimer A, Folster JP, Walker M, Dahourou GA, Batra DG, Drug-resistance mechanisms in Vibrio cholerae O1 outbreak strain, Haiti, 2010. Emerg Infect Dis. 2011;17:2151–4 . DOIPubMed
5. Steenland MW, Joseph GA, Lucien MA, Freeman N, Hast M, Nygren BL, Laboratory-confirmed cholera and rotavirus among patients with acute diarrhea in four hospitals in Haiti, 2012–2013. Am J Trop Med Hyg. 2013;89:641–6. DOIPubMed
6. Katz LS. Petkau A, Beaulaurier J, Tyler S, Antonova ES, Turnsek MA, et al., Evolutionary dynamics of Vibrio cholerae O1 following a single-source introduction to Haiti. MBio. 2013;4:e00398-13. doi:
7. Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother. 2012;67:2640–4. DOIPubMed
8. Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods.2005;63:219–28. DOIPubMed
9. Young HK, Amyes SG. Plasmid trimethoprim resistance in Vibrio cholerae: migration of the type I dihydrofolate reductase gene out of theEnterobacteriaceae. J Antimicrob Chemother. 1986;17:697–703. DOIPubMed
10. Call DR, Singer RS, Meng D, Broschat SL, Orfe LH, Anderson JM, blaCMY-2–positive IncA/C plasmids from Escherichia coli and Salmonella entericaare a distinct component of a larger lineage of plasmids. Antimicrob Agents Chemother. 2010;54:590–6. DOIPubMed

Table

• Table. Susceptibility, resistance genes, and plasmids associated with Haiti Vibrio cholerae outbreak isolates

Technical Appendix

• Technical Appendix. Additional methods and comparison of plasmid p2012EL-2176 and plasmid pAR060302.  121 KB

Suggested citation for this article: Folster JP, Katz L, McCullough A, Parsons MB, Knipe K, Sammons SA, et al. Multidrug-resistant IncA/C plasmid in Vibrio cholerae from Haiti [letter]. Emerg Infect Dis [Internet]. 2014 Nov [date cited]. http://dx.doi.org/10.3201/eid2011.140889

DOI: 10.3201/eid2011.140889