WU Polyomavirus in Respiratory Epithelial Cells from Lung Transplant Patient with Job Syndrome - Volume 21, Number 1—January 2015 - Emerging Infectious Disease journal - CDC

full-text ►

WU Polyomavirus in Respiratory Epithelial Cells from Lung Transplant Patient with Job Syndrome - Volume 21, Number 1—January 2015 - Emerging Infectious Disease journal - CDC

Volume 21, Number 1—January 2015

Dispatch

WU Polyomavirus in Respiratory Epithelial Cells from Lung Transplant Patient with Job Syndrome

On This Page

• The Study
• Conclusions
• Suggested Citation

Figures

• Figure 1
• Figure 2

Downloads

• PDF[410 KB - 4 pgs]
• RIS[TXT - 2 KB]

Erica A. Siebrasse, Diana V. Pastrana, Nang L. Nguyen, Annie Wang, Mark J. Roth, Steven M. Holland, Alexandra F. Freeman, John McDyer, Christopher B. Buck, and David Wang 

Author affiliations: Washington University School of Medicine, St. Louis, Missouri, USA (E.A. Siebrasse, N.L. Nguyen, A. Wang, D. Wang); National Institutes of Health, Bethesda, Maryland, USA (D.V. Pastrana, M.J. Roth, S.M. Holland, A.F. Freeman, C.B. Buck); University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania, USA (J. McDyer)

Suggested citation for this article

Abstract

We detected WU polyomavirus (WUPyV) in a bronchoalveolar lavage sample from lungs transplanted into a recipient with Job syndrome by using immunoassays specific for the WUPyV viral protein 1. Co-staining for an epithelial cell marker identified most WUPyV viral protein 1–positive cells as respiratory epithelial cells.

WU polyomavirus (WUPyV) was discovered in a child with pneumonia in 2007 (1). Subsequent studies showed that WUPyV infection is common (2–4), and viral DNA can be detected in a variety of specimen types, including respiratory tract secretions (5). However, the virus has yet to be associated with any disease, and the specific cell type(s) infected by WUPyV has not been identified. Other human polyomaviruses are known pathogens, which typically cause disease in the context of immunosuppression (6–8).

Job syndrome is an immune disorder characterized by eczematoid dermatitis, recurrent skin and pulmonary infections, increased levels of IgE, and impaired T and B cell memory (9). This disorder is caused by dominant-negative mutations in the STAT3 gene (9). We report WUPyV cell tropism in lungs transplanted into a recipient with Job syndrome.

Ms. Siebrasse is a graduate student at Washington University, St. Louis, Missouri. Her research interests focus on discovery and characterization of novel polyomaviruses.

Acknowledgments

We thank Kenneth N. Olivier and Erika Crouch for help with interpretation of the slides, Peter C. FitzGerald for assistance with the bioinformatics analysis, and Patti Fetsch for immunocytochemical staining of SV40.

Experimental support was provided by the Hybridoma Facility of the Rheumatic Diseases Core Center. This study was supported by the National Institute of Arthritis and Musculoskeletal and Skin Diseases, NIH (award no. P30AR048335); the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH; grant R21AI095922 to D.W.; the Children’s Discovery Institute at Washington University in St. Louis; and the NIH Intramural Research Program, with support from the Center for Cancer Research and National Institute of Allergy and Infectious Diseases. E.A.S. was supported by the Department of Defense through the National Defense Science and Engineering Graduate Fellowship Program.

References

1. Gaynor AM, Nissen MD, Whiley DM, Mackay IM, Lambert SB, Wu G, Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathog. 2007;3:e64 . DOIPubMed
2. Nguyen NL, Le BM, Wang D. Serologic evidence of frequent human infection with WU and KI polyomaviruses. Emerg Infect Dis. 2009;15:1199–205 .DOIPubMed
3. Kean JM, Rao S, Wang M, Garcea RL. Seroepidemiology of human polyomaviruses. PLoS Pathog. 2009;5:e1000363. DOIPubMed
4. Neske F, Prifert C, Scheiner B, Ewald M, Schubert J, Opitz A, High prevalence of antibodies against polyomavirus WU, polyomavirus KI, and human bocavirus in German blood donors. BMC Infect Dis. 2010;10:215. DOIPubMed
5. Babakir-Mina M, Ciccozzi M, Perno CF, Ciotti M. The novel KI, WU, MC polyomaviruses: possible human pathogens? New Microbiol. 2011;34:1–8.PubMed
6. Knowles WA. Discovery and epidemiology of the human polyomaviruses BK virus (BKV) and JC virus (JCV). Adv Exp Med Biol. 2006;577:19–45.DOIPubMed
7. Kazem S, van der Meijden E, Kooijman S, Rosenberg AS, Hughey LC, Browning JC, Trichodysplasia spinulosa is characterized by active polyomavirus infection. J Clin Virol. 2012;53:225–30. DOIPubMed
8. Gjoerup O, Chang Y. Update on human polyomaviruses and cancer. Adv Cancer Res. 2010;106:1–51. DOIPubMed
9. Sowerwine KJ, Holland SM, Freeman AF. Hyper-IgE syndrome update. Ann N Y Acad Sci. 2012;1250:25–32. DOIPubMed
10. Schowalter RM, Pastrana DV, Pumphrey KA, Moyer AL, Buck CB. Merkel cell polyomavirus and two previously unknown polyomaviruses are chronically shed from human skin. Cell Host Microbe. 2010;7:509–15. DOIPubMed
11. Siebrasse EA, Nguyen NL, Smith C, Simmonds P, Wang D. Immunohistochemical detection of KI polyomavirus in lung and spleen. Virology.2014;468–470C:178–184.
12. White MK, Safak M, Khalili K. Regulation of gene expression in primate polyomaviruses. J Virol. 2009;83:10846–56. DOIPubMed

Figures

• Figure 1. WU polyomavirus antigen in bronchoalveolar lavage specimens from lungs transplanted into a recipient (28-year-old woman) with Job syndrome. Immunohistochemical analysis of 293T cells transfected with pDEST26-WU–virus protein 1 and...
• Figure 2. WU polyomavirus antigen in respiratory epithelial cells from lungs transplanted into a recipient (28-year-old woman) with Job syndrome. Immunofluorescence of 293T cells transfected with pDEST26-WU–virus protein 1 and stained...

Suggested citation for this article: Siebrasse EA, Pastrana DV, Nguyen NL, Wang A, Roth MJ, Holland SM, et al. WU polyomavirus in respiratory epithelial cells from lung transplant patient with Job syndrome. Emerg Infect Dis [Internet]. 2015 Jan [date cited]. http://dx.doi.org/10.3201/eid2101.140855

DOI: 10.3201/eid2101.140855