lunes, 23 de marzo de 2015

Molecular Characterization of Echinococcus granulosus Sensu Lato fr... - PubMed - NCBI

Molecular Characterization of Echinococcus granulosus Sensu Lato fr... - PubMed - NCBI



 2015 Mar 11;10(3):e0118509. doi: 10.1371/journal.pone.0118509. eCollection 2015.

Molecular Characterization of Echinococcus granulosus Sensu Lato from Farm Animals in Egypt.

Abstract

Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Direct sequencing of amplicons indicated the presence of Echinococcus canadenesis (G6 genotype) in 26 of 28 camel cysts, 3 of 7 sheep cysts and the 2 buffalo derived cysts. In contrast, Echinococcus granulosus sensu stricto (G1 genotype) was detected in one cyst from a camel and 4 of 7 cysts from sheep, whereas Echinococcus ortleppi (G5 genotype) was detected in one cyst from a camel. This is the first identification of E. ortleppi in Egypt.

PMID:
 
25760944
 
[PubMed - in process] 
PMCID:
 
PMC4356597
 
Free PMC Article

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