Development and application of a gp60-based typing assay for Cryptosporidium viatorum. - PubMed - NCBI
Development and application of a gp60-based typing assay for Cryptosporidium viatorum.
Abstract
The apicomplexan intestinal parasite Cryptosporidium takes a major toll on human and animal health and is a frequent cause of waterborne outbreaks. Several species and genotypes can infect humans, including Cryptosporidium viatorum, which, to date, has only been found in humans. Molecular characterization of Cryptosporidium spp., critical to epidemiological analyses, is commonly based on gp60 gene analysis, which appears to require bespoke species- or group-specific PCR primers due to extensive genetic diversity across the genus. In this study, we amplified, sequenced, and characterized the gp60 gene of C. viatorum for the first time. Moreover, a gp60 typing assay for this species was developed, validated, and applied to 27 isolates originating from Asia, Africa, and Central America. One single subtype family, XVa, was identified containing multiple alleles. Copyright © 2015, American Society for Microbiology. All Rights Reserved.
- PMID:
- 25832304
- [PubMed - as supplied by publisher]
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